Stimulation of muscle anabolism by resistance exercise and ingestion of leucine plus protein.

Leucine is known to stimulate muscle protein synthesis and anabolism. However, evidence for the efficacy of additional leucine to enhance the response of muscle anabolism to resistance exercise and protein ingestion is unclear. Thus, we investigated the response of net muscle protein balance to ingestion of additional leucine with protein in association with resistance exercise. Two groups of untrained subjects performed an intense bout of leg resistance exercise following ingestion of 1 of 2 drinks: flavored water (PL); or 16.6 g of whey protein + 3.4 g of leucine (W+L). Arteriovenous amino acid balance across the leg was measured to assess the anabolic response of muscle in each group. Arterial amino acid concentrations increased in response to ingestion of W+L. Amino acid concentrations peaked between 60 and 120 min after ingestion, and then declined to baseline values. Valine concentration decreased to levels significantly lower than baseline. Net balance of leucine, threonine, and phenylalanine did not change following PL ingestion, but increased and remained elevated above baseline for 90-120 min following W+L ingestion. Leucine (138 +/- 37 and -23 +/- 23 mg), phenylalanine (58 +/- 28 and -38 +/- 14 mg), and threonine (138 +/- 37 and -23 +/- 23 mg) uptake was greater for W+L than for PL over the 5.5 h following drink ingestion. Our results indicate that the whey protein plus leucine in healthy young volunteers results in an anabolic response in muscle that is not greater than the previously reported response to whey protein alone.

PMID: 19370045 [PubMed - in process]

Seems odd why they didn't have a third drink that would've been just whey (16.6g) ??

health4ni wrote:Seems odd why they didn't have a third drink that would've been just whey (16.6g) ??

.. Or a 4th drink of just Leucine?

Oh and as always they've used untrained subjects - I want to see a study using trained subjects

^^ true. But I think it's been proven that leucine on it's own (away from other aminos) is rather useless.

They always seems to do studies on untrained people

health4ni wrote:^^ true. But I think it's been proven that leucine on it's own (away from other aminos) is rather useless.

They always seems to do studies on untrained people

Hmmmm I dont think so Scott. Its been proven that Leu on its own also works.

Ill try and dig out the studies.

I wonder why someone / somebody doesnt do a decent study on trained individuals!

I mean it cant be hard.

And then just do it for the most popular supplements over like 6 weeks [which is long enough]

I reckon it woulddent a lot of supplement sales!

kp1512 wrote:

health4ni wrote:^^ true. But I think it's been proven that leucine on it's own (away from other aminos) is rather useless.

They always seems to do studies on untrained people

Hmmmm I dont think so Scott. Its been proven that Leu on its own also works.

Ill try and dig out the studies.

A few studies would be good. I was under the impression it was dependent on other aminos.

health4ni wrote:

kp1512 wrote:

health4ni wrote:^^ true. But I think it's been proven that leucine on it's own (away from other aminos) is rather useless.

They always seems to do studies on untrained people

Hmmmm I dont think so Scott. Its been proven that Leu on its own also works.

Ill try and dig out the studies.

A few studies would be good. I was under the impression it was dependent on other aminos.

It is. Leucine will cause PS within the limiting factors present ie eaa.

Ader wrote:

health4ni wrote:Seems odd why they didn't have a third drink that would've been just whey (16.6g) ??

.. Or a 4th drink of just Leucine?

Oh and as always they've used untrained subjects - I want to see a study using trained subjects

Yeah this gets on my tits, if it's down to money they can't get more people then just make each group smaller? It would make the results far more interesting. I mean what does this tell us that whey and leucine is better than just vimto?? Yeah great thanks for that.... n00bs.

As for using untrained individuals I think that's just down to lazy recruiting?

Would you want to ruin a few weeks worth of training for a study though? Could be why they use the untrained.

I suspect the actual reason is more that average joe public is untrained and these studies are for medical purposes, not sporting.

Alex wrote:Would you want to ruin a few weeks worth of training for a study though? Could be why they use the untrained.

I suspect the actual reason is more that average joe public is untrained and these studies are for medical purposes, not sporting.

lets not forget the damage also it may due to the industry

Imagine doing a study on Gutamine on trained individuals taken orally not IV...would be the first one!